Vitamin B1


Test Information

Test Name

Vitamin B1

Synonyms

Thiamine; RBC Transketolase

Preferred Specimen

Heparin (non gel)

Testing Location

Referral

Turn Around Time

3 weeks


Collection Information

Main Lab Only

Yes

Availability

Testing will only be performed if approved by the chemical pathologist, or paid $70.00

Preparation

  • Send to lab ASAP

Processing Information

Registration Code

THIA

Notes

  • DO NOT share sample; Dedicated tube required
  • Process ASAP

The red blood cells must be washed, haemolysed and frozen before they are 4 hours old (post venepuncture). Samples for analysis of thiamine are prepared by washing the red cells with isotonic 0.9% saline, preparing a red cell lysate and freezing.

METHOD FROM CHL WEBSITE

  1. Centrifuge the sample at 3000 rpm for 5 minutes.
  2. After centrifugation use a pasteur pipette to remove and discard the plasma and buffy coat layer.
  3. The red cells are washed by adding an equivalent volume of isotonic saline (9 g/L NaCl) and mixing thoroughly.
  4. Centrifuge the sample at 3000 rpm for 5 min.
  5. Label a plastic tube suitable for transport, with the patient surname and laboratory number.
  6. After centrifugation use a pasteur pipette to remove the top saline layer and 1 mm of red cells. Discard, leaving behind the washed red cells.
  7. To the labelled tube add 1 mL of washed packed red blood cells (RBC) from the middle of the washed cells.
  8. Using the same pipette tip, draw up 1 mL of distilled water and add this to the cells in the labelled tube.
  9. Stopper, mix thoroughly by rolling the haemolysate around the inside of the tube.
  10. Place the tube inside a plastic bag and freeze.
  11. When frozen, send to laboratory.

Site Specific Notes

- Tauranga

Send directly to referral laboratory

- Rotorua

Test not available


Methodology

Interpretations

View Interpretations

Contact Information

laura.yau@pathlab.co.nz

External Referral Information

Referral Location

Canterbury Health Laboratory (via WHL)

Send Away Transport

Frozen

External Lab Website