Testing will only be performed if approved by the chemical pathologist, or paid $70.00
Send to lab ASAP
DO NOT share sample; Dedicated tube required
The red blood cells must be washed, haemolysed and frozen before they are 4 hours old (post venepuncture). Samples for analysis of thiamine are prepared by washing the red cells with isotonic 0.9% saline, preparing a red cell lysate and freezing.
METHOD FROM CHL WEBSITE
Centrifuge the sample at 3000 rpm for 5 minutes.
After centrifugation use a pasteur pipette to remove and discard the plasma and buffy coat layer.
The red cells are washed by adding an equivalent volume of isotonic saline (9 g/L NaCl) and mixing thoroughly.
Centrifuge the sample at 3000 rpm for 5 min.
Label a plastic tube suitable for transport, with the patient surname and laboratory number.
After centrifugation use a pasteur pipette to remove the top saline layer and 1 mm of red cells. Discard, leaving behind the washed red cells.
To the labelled tube add 1 mL of washed packed red blood cells (RBC) from the middle of the washed cells.
Using the same pipette tip, draw up 1 mL of distilled water and add this to the cells in the labelled tube.
Stopper, mix thoroughly by rolling the haemolysate around the inside of the tube.